Digestive Disease Week (DDW 2024)

  • 18-21 May 2024
  • Washington, DC, United States(Hybrid)

Description

Digestive Disease Week (DDW 2024) is a conference that covers topics such as:

  • Liver disease
  • Diagnosis and treatment of gastrointestinal tract and liver disorders
  • Diseases and functions of the alimentary tract
  • Gastrointestinal Endoscopy

Digestive Disease Week (DDW 2024) brings together researchers and physicians in the fields of hepatology, gastroenterology, gastrointestinal surgery and endoscopy.

Venue

  • Walter E. Washington Convention Center , 801 Mount Vernon Place, Washington, District of Columbia, United States
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More Details

Prices:
0-685 US Dollar (Estimated)
Early registration date: 13 Mar 2024
Exhibition:
Included
Organizer:
AGA Institute - American Gastroenterological Association
Website:

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Important

Please, check "Digestive Disease Week (DDW)" official website for possible changes, before making any traveling arrangements

Event Categories

Health & Medicine: Gastroenterology, Hepatology

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Backgrounds: Angiogenesis plays an important role in the development of liver fibrosis and portal hypertension. Long non-coding RNAs (lncRNAs) are involved in the liver diseases and angiogenesis.

Objective: This study aimed to investigate the effect of lncRNA-COX-2 in the progress of liver fibrosis, and the underlying mechanisms were also studied.

Methods: Thirty-six SPF Bal B/C mice were randomly assigned into 3 groups with 12 mice in each group. The control group received injection of normal saline (100 µl, twice a week); the CCl4-2M group given intraperitoneal injection of carbon tetrachloride (CCl4) for 2 months; the CCl4-3M group given intraperitoneal injection of CCl4 for 3 months. The hepatic fibrosis was assessed by the hepatic fibrotic areas. Quantitative real-time PCR was performed to evaluate the expressions of lncRNA-COX-2 and COX-2 in the liver tissue, and immunohistochemistry staining was used to detect COX-2 in the liver tissue. Correlation of lncRNA-COX-2 and COX-2 with liver fibrotic areas was completed by using Pearson correlation analysis. In vitro, the EOMA endothelial cells were treated with lipopolysaccharide (LPS) for 6 hours, and lncRNA-COX-2, COX-2, VEGF and VEGFR-2 were evaluated by quantitative real-time PCR and immunohistochemistry staining.

Results:

Extensive nodular formation was observed in the liver after the treatment with CCl4. Compared with the control group, fibrotic areas of liver tissues in the CCl4-2M group and CCl4-3M group were increased by 4 times and 9 times, respectively. The CD31 positive areas and vascular areas were significantly increased in cirrhotic livers. Additionally, up-regulations of COX-2 mRNA and COX-2 protein were observed in both the CCl4-2M group and the CCl4-3M group, compared with the control group. Moreover, the level of lncRNA-COX-2 in the CCl4-2M group and CCl4-3M group were nearly 2 times higher than that in the control group. Meanwhile, the expression of COX-2 and lncRNA-COX-2 were positively correlated with the liver fibrotic areas. (R=0.801, P<0.05; R=0.593, P<0.05). Besides, a positive correlation between the expression of COX-2 mRNA and lncRNA-COX-2 was  displayed in cirrhotic liver (R=0.667, P<0.05). And there also was a positive correlation between the CD31 positive areas and lncRNA-COX-2 in cirrhotic liver (R=0.605, P<0.05). In vitro, the gene expression of lncRNA-COX-2, COX-2, VEGF and VEGFR-2 were remarkably increased in LPS-treated EOMA cells. Consistently, the protein expression of COX-2 and VEGF were also obviously increased in LPS-treated EOMA cells.

Conclusions: Expressions of COX-2 and lncRNA-COX-2 increase in the progress of liver fibrosis. LncRNA-COX-2 may exacerbate liver fibrosis via increasing the inhtraheaptic angiogenesis. And the neoangiogenic effect of lncRNA-COX-2 may attribute to its up-regulation of COX-2, VEGF and VEGFR-2.